The purpose of this workshop is to introduce systems science of bio-navigation. We are trying to develop the foundation of research scheme that integrates robotics, data science, ecology, and neuroscience, with the theme of navigation. In navigation, animals as well as humans properly and promptly perform the following: the acquisition of dynamically-changing information from external and internal environment, the choice of route and destination based on the information, and the behavioral regulation to reach the destination, all of which can be modified by their memory, emotions etc. Thus, through the study of navigation, we can also analyze a variety of brain functions. In this workshop, we will introduce the incredible abilities of animals during navigation, and ongoing research projects understanding and applying them.